In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens.
Chow RD, Wang G, Ye L, Codina A, Kim HR, Shen L, Dong MB, Errami Y, Chen S
Nat Methods. 2019 May;16(5):405-408. doi: 10.1038/s41592-019-0371-5. Epub 2019 Apr 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123359 | pRC10-EFS-huLbCpf1-2A-Blast-WPRE | Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection. |
| 123360 | pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE | Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance. |
| 123361 | pRG01-MCAP_MET | pRG01 with barcoded MCAP_Met crRNA array library cloned in, targeting combinations of metastasis drivers. |
| 123362 | pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE | Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance and Firefly luciferase. |
| 123363 | pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE | Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP. |