Engineering of CRISPR-Cas12b for human genome editing.

Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F
Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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Search table:

ID	Plasmid	Purpose
158615	TwinStrep-SUMO-NLS-BhCas12b_v4	For protein expression of BhCas12b_v4
122448	pZ148-BhCas12b-sgRNA-scaffold	Expresses sgRNA for BhCas12b in mammalian cells
122447	pZ147-BvCas12b-sgRNA-scaffold	Expresses sgRNA for BvCas12b in mammalian cells
122446	pZ149-pcDNA3-BhCas12b_v4	Expresses BhCas12b_v4 in mammalian cells
122445	pZ143-pcDNA3-BvCas12b	Expresses BvCas12b in mammalian cells