Engineering of CRISPR-Cas12b for human genome editing.
Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, Makarova KS, Koonin EV, Zhang F
Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122445 | pZ143-pcDNA3-BvCas12b | Expresses BvCas12b in mammalian cells |
| 122446 | pZ149-pcDNA3-BhCas12b_v4 | Expresses BhCas12b_v4 in mammalian cells |
| 122447 | pZ147-BvCas12b-sgRNA-scaffold | Expresses sgRNA for BvCas12b in mammalian cells |
| 122448 | pZ148-BhCas12b-sgRNA-scaffold | Expresses sgRNA for BhCas12b in mammalian cells |
| 158615 | TwinStrep-SUMO-NLS-BhCas12b_v4 | For protein expression of BhCas12b_v4 |