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Molecular Characterization of Adenylyl Cyclase Complex Proteins Using Versatile Protein-Tagging Plasmid Systems in Cryptococcus neoformans.

So YS, Yang DH, Jung KW, Huh WK, Bahn YS
J Microbiol Biotechnol. 2017 Feb 28;27(2):357-364. doi: 10.4014/jmb.1609.09036. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
92081YSBE 508 : pNEO_GFPPlasmid for tagging proteins in Cryptococcus neoformans; GFP tag, Neo selection
92082YSBE 516 : pNEO_4xFLAGPlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, Neo selection
92083YSBE 517 : pNEO_6xHAPlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, Neo selection
92084YSBE 524 : pNEO_mCherryPlasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, Neo selection
92085YSBE 557 : pNAT_GFPPlasmid for tagging proteins in Cryptococcus neoformans; GFP tag, NAT selection
92086YSBE 558 : pNAT_4xFLAGPlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, NAT selection
92087YSBE 559 : pNAT_6xHAPlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, NAT selection
92088YSBE 596 : pNAT_mCherryPlasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, NAt selection
92089YSBE 608 : pHYG_GFPPlasmid for tagging proteins in Cryptococcus neoformans; GFP tag, HYG selection
92090YSBE 731 : pHYG_4xFLAGPlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selection
92091YSBE 610 : pHYG_6xHAPlasmid for tagging proteins in Cryptococcus neoformans; 6xHA tag, HYG selection
92092YSBE 628 : pHYG_mCherryPlasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, HYG selection
122434YSBE415: pTOP_ACA1_Vc_HOG1 terminatorPlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein
122435YSBE416: pTOP_CAC1_Vn_HOG1 terminatorPlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein
122700YSBE418:pNAT_ACA1_Vc_HOG1 terminatorPlasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein
122701YSBE420:pNEO_CAC1_Vn_HOG1 terminatorPlasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

Antibodies from Article