A CRISPR Interference Platform for Efficient Genetic Repression in Candida albicans.
Wensing L, Sharma J, Uthayakumar D, Proteau Y, Chavez A, Shapiro RS
mSphere. 2019 Feb 13;4(1). pii: 4/1/e00002-19. doi: 10.1128/mSphere.00002-19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122377 | pRS143 | CRISPRi plasmid for use in Candida albicans. Contains dCas9 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail) |
| 122378 | pRS159 | CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and the sgRNA cloning site (SNR52 promoter, PacI sites, sgRNA tail) |
| 122379 | pRS263 | CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon |
| 122380 | pRS267 | CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon |