Reconstitution and analyses of RNA interactions with eukaryotic translation initiation factors and ribosomal preinitiation complexes.

Liu X, Schuessler PJ, Sahoo A, Walker SE
Methods. 2019 Mar 26. pii: S1046-2023(18)30395-5. doi: 10.1016/j.ymeth.2019.03.024. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
122248	pSW149	pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.
122295	pSW104	For run-off transcription of yeast RPL41A mRNA with and without an 82 nucleotide poly(A) tail.