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Interrogation of genome-wide, experimentally dissected gene regulatory networks reveals mechanisms underlying dynamic cellular state control

Tan X, Worley J, Turunen M, Wong K, Fernández EC, Paull E, Jones S, Wang J, Noh H, Salvatori B, Chavez A, Califano A
bioRxiv 2021.06.28.449297 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
122205lenti_dCas9-KRAB-MeCP2Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.
122267PB-TRE-dCas9-KRAB-MeCP2SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression
140690pLX-TRE-dCas9-KRAB-MeCP2-BSDInducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

Antibodies from Article