Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds.
Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher JE, Brangwynne CP
Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 10.1016/j.cell.2018.10.048.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122147 | M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1 | Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins. |
| 122148 | M23: pHR-SFFVp-FUSN::mCherry::SspB | N terminal domain of FUS (1-214) tagged with SspB and mCherry. |
| 122149 | M43: pHR-SFFVp-iLID::EGFP::FTH1 | Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins. |
| 122668 | M30: pHR-SFFVp- HNRNPA1C::mCherry::SspB | C terminal domain of hnRNPA1 (186-320) tagged with SspB and mCherry. |
| 122669 | M31: pHR-SFFVp- TDP-43C::mCherry::SspB | C terminal domain of TDP43 (218-414) tagged with SspB and mCherry. |