Accelerated genome engineering of Pseudomonas putida by I-SceI-mediated recombination and CRISPR-Cas9 counterselection.
Wirth NT, Kozaeva E, Nikel PI
Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13396.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122086 | pGNW2 | suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells |
| 122088 | pGNW4 | suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells |
| 122093 | pGNW6 | suicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cells |
| 122094 | pSEVA6213S | constitutive expression of I-SceI for genome engineering in Pseudomonas |
| 122095 | pSEVA1213S | constitutive expression of I-SceI for genome engineering in Pseudomonas |
| 122096 | pS448∙CsR | CRISPR/Cas9 counterselection in Gram-negative bacteria |