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The crystal structure of the naturally split gp41-1 intein guides the engineering of orthogonal split inteins from cis-splicing inteins.

Beyer HM, Mikula KM, Li M, Wlodawer A, Iwai H
FEBS J. 2019 Oct 30. doi: 10.1111/febs.15113. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
121910pSADuet148Charge-introduced NpuDnaBΔ283ΔC39 intein
121911pHBDuet259NpuDnaBΔ283ΔC39 intein
121912pHBBAD113Charge-Introduced NpuDnaBC39 intein
121913pHBDuet139Charge-Introduced NpuDnaB mini-intein
121914pHBDuet140Orthogonal NpuDnaB mini-intein intein
121915pHBDuet116NpuDnaBΔ290ΔC39 intein
121916pHBBAD168Oth-NpuDnaBC39 intein

Antibodies from Article