Conversion of MyoD to a neurogenic factor: binding site specificity determines lineage.
Fong AP, Yao Z, Zhong JW, Johnson NM, Farr GH 3rd, Maves L, Tapscott SJ
Cell Rep. 2015 Mar 31;10(12):1937-46. doi: 10.1016/j.celrep.2015.02.055. Epub 2015 Mar 19.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122053 | pRRLSIN.cPPT.PGK.MyoD.WPRE | Lentiviral expression of MyoD |
| 122054 | pRRLSIN.cPPT.PGK.NeuroD2.WPRE | Lentiviral expression of NeuroD2 |
| 122055 | pRRLSIN.cPPT.PGK.MD(ND2bHLH).WPRE | Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain |
| 122056 | pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE | Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P |