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Metagenomic engineering of the mammalian gut microbiome in situ.

Ronda C, Chen SP, Cabral V, Yaung SJ, Wang HH
Nat Methods. 2019 Feb;16(2):167-170. doi: 10.1038/s41592-018-0301-y. Epub 2019 Jan 14. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
122568pGT-Ah1MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)
122569pGT-Ah2MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)
122570pGT-Ah3MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)
122572pGT-Ah5MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)
122573pGT-Ah6MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)
122574pGT-Ah7MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)
122576pGT-Ah9MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)
122577pGT-Ah10MAGIC donor plasmid (Himar transposon with tetQ/sfGFP payload)
122579pGT-B1MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)
122580pGT-B2MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)
122581pGT-B3MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)
122582pGT-S1MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)
122583pGT-S2MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)
122587pGT-Kh2MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

Antibodies from Article