Generating stable cell lines with quantifiable protein production using CRISPR/Cas9-mediated knock-in.
Lo CA, Greben AW, Chen BE
Biotechniques. 2017 Apr 1;62(4):165-174. doi: 10.2144/000114534.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121448 | ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP | To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations |
| 165043 | RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR | Repair template for CAS9 complex genome editing |