Generation of conditional auxin-inducible degron (AID) cells and tight control of degron-fused proteins using the degradation inhibitor auxinole.
Yesbolatova A, Natsume T, Hayashi KI, Kanemaki MT
Methods. 2019 Apr 23. pii: S1046-2023(18)30331-1. doi: 10.1016/j.ymeth.2019.04.010.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121178 | pMK344 (Hygro-P2A-mAID) | N-terminal tagging |
| 121179 | pMK345 (Hygro-P2A-mAID-mClover) | N-terminal tagging |
| 121180 | pMK346 (Hygro-P2A-mAID-mCherry) | N-terminal tagging |
| 121181 | pMK347 (BSD-P2A-mAID) | N-terminal tagging |
| 121182 | pMK348 (BSD-P2A-mAID-mClover) | N-terminal tagging |
| 121183 | pMK349 (BSD-P2A-mAID-mCherry) | N-terminal tagging |
| 121184 | pMK364 (CMV-OsTIR1-loxP-PURO-loxP) | OsTIR1 introduction |
| 121185 | pMK365 (Tet-OsTIR1-loxP-PURO-loxP) | OsTIR1 introduction |
| 121186 | pMK375 (Hygro-P2A-Stag-3FLAG) | N-terminal tagging |
| 121187 | pMK376 (Hygro-P2A-mClover) | N-terminal tagging |
| 121188 | pMK377 (Hygro-P2A-mCherry2) | N-terminal tagging |
| 121189 | pMK388 (mClover-BSD) | C-terminal tagging |
| 121190 | pMK389 (mCherry-BSD) | C-terminal tagging |
| 121191 | pMK390(Stag-3FLAG-BSD) | C-terminal tagging |
| 121192 | pMK391 (mAID-BSD) | C-terminal tagging |
| 121193 | pMK392 (mAID-mClover-BSD) | C-terminal tagging |
| 121194 | pMK393 (mAID-mCherry-BSD) | C-terminal tagging |
| 121195 | pMK394 (mAID-Nluc-Neo) | C-terminal tagging |
| 121196 | pMK401 (Hygro-P2A-mAID-Nluc) | N-terminal tagging |
| 121197 | pMK407 (BSD-P2A-mAID-Nluc) | N-terminal tagging |
| 121198 | pMK408 (mAID-Nluc Hygro) | C-terminal tagging |
| 121199 | pMK409 (mAID-Nluc BSD) | C-terminal tagging |