Timo Otonkoski Lab: Otonkoski plasmids 2018

Unpublished

Plasmids from Article

ID	Plasmid	Purpose
121169	CAG-FNLS-T2A-EGFP-ires-puro	CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
121170	CAG-ABEmax-P2A-EGFP-ires-puro	CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
121171	CAG-xFNLS-T2A-EGFP-ires-puro	CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
121173	FU_tetO_dCas9-VP192-T2A-EGFP	Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.
121174	pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro	Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
121175	pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro	Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines
121176	lentiCRISPRv2_PROX1_1+3	Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
121177	lentiCRISPRv2_PROX1_1+4	Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.