Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
Hayashi A, Tanaka K
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121144 | pAH233 | Expression of gRNA with BbsI placeholder for target cloning |
| 121145 | pAH235 | Cas9 expression controlled by the nmt41 promoter |
| 121146 | pAH237 | Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast |
| 121147 | pAH243 | Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast |