CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization.
Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, La Russa M, Qi LS
Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121513 | pHR-PGK-ABI-dCas9-P2A-mcherry | expression of AB1-fused-dcas9 plasmids |
| 121532 | pHR-SFFV-PYL1-sfGFP-Emerin | expression of PYL-SfGFP-Emerin, lentivirus vector |
| 122177 | pHR-PGK-PYL1-sfGFP-Coilin | expression of PYL1-SfGFP-Coilin, lentivirus vector |
| 167011 | pHR-TRE3G-PYL1-sfGFP-PML | expression of PYL1-SfGFP-PML, lentivirus vector |
| 169448 | pHR-TRE3G-dCas9-HaloTag | expression of dCas9-HaloTag |
| 169449 | pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1 | expression of TRF1-mcherrry and sgRNA targeting telomeres |
| 169450 | pHR-U6-sgRNA-CMV-puro | backbone for sgRNA expression |
| 169451 | pHR-TRE3G-dCas9-EGFP-HaloTag | expression of dCas9-EGFP-HaloTag |