Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched.
Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers BR, Cho W, Cheng Y, Beachy PA
Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 10.1016/j.cell.2018.10.026. Epub 2018 Nov 8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120886 | pVLAD6-mmPtch1-B-SBP | BacMam vector for Ptch1-B expression |
| 120887 | pVLAD6-mmPtch1-B-NNQ-TEV-SBP | BacMam vector for Ptch1-B NNQ variant expression |
| 120888 | pcDNA-h-mmPtch1-B | Expresses Ptch1-B for function assays |
| 120889 | pcDNA-h-mmPtch1-FL | Expresses wildtype Ptch1 for function assays |
| 120902 | pcDNA-h-mmPtch1-B-HA | Expresses Ptch1-B for function assays |
| 120903 | pcDNA-h-mmPtch1-B-HA-GFP | Expresses Ptch1-B for function assays |
| 120904 | pcDNA-h-mmPtch1-B-Y233C-HA | Expresses Ptch1-B (Y233C) for function assays |
| 120905 | pcDNA-h-mmPtch1-B-L234C-HA | Expresses Ptch1-B (L234C) |
| 120906 | pcDNA-h-mmPtch1-B-L235C-HA | Expresses mmPtch1-B (L235C) |
| 120907 | pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP | Expresses Ptch1-B (I766FV111F) |
| 120908 | pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP | Expresses Ptch1-B (V111FL114FW115A) |
| 120909 | pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP | Expresses Ptch1-B (I766FV111FL114F) |
| 120910 | pcDNA-h-mmPtch1-B-HA-TagBFP | Expresses BFP tagged Ptch1-B |
| 120911 | pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFP | Expresses BFP tagged Ptch1-B (V111FL114FI766F) |
| 120921 | pcDNA-h-mmPtch1-FL-TagBFP | Expresses BFP tagged Ptch1 |
| 121135 | pHTSHP-Smt-IIShhN | Expresses N-terminal SUMO tagged II-ShhN in bacteria |
| 121136 | pHTSHP-ShhN-Cys | Expresses ShhN with a C-terminal cysteine residues in bacteria |
| 121164 | pcDNA-h-mmPtch1-B-NNQ-HA-GFP | Expresses mmPtch1-B NNQ variant |