An Ultrasensitive Calcium Reporter System via CRISPR-Cas9-Mediated Genome Editing in Human Pluripotent Stem Cells.
Jiang Y, Zhou Y, Bao X, Chen C, Randolph LN, Du J, Lian XL
iScience. 2018 Oct 12;9:27-35. doi: 10.1016/j.isci.2018.10.007.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120896 | AAVS1-Puro-CAG-GCaMP6s | Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene. |