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Overcoming component limitations in synthetic biology through transposon-mediated protein engineering.

Atkinson JT, Wu B, Segatori L, Silberg JJ
Methods Enzymol. 2019;621:191-212. doi: 10.1016/bs.mie.2019.02.014. Epub 2019 Apr 2. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120863pMT-P1Encodes permuteposon P1 for MuA transposase-mediated circular permutation
120864pMT-P2Encodes permuteposon P2 for MuA transposase-mediated circular permutation
120865pMT-P3Encodes permuteposon P3 for MuA transposase-mediated circular permutation
120866pMT-P4Encodes permuteposon P4 for MuA transposase-mediated circular permutation

Antibodies from Article