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Use of mCherry Red fluorescent protein for studies of protein localization and gene expression in Clostridium difficile.

Ransom EM, Ellermeier CD, Weiss DS
Appl Environ Microbiol. 2015 Mar;81(5):1652-60. doi: 10.1128/AEM.03446-14. Epub 2014 Dec 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120812pDSW1728E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)
120813pRAN473E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt), multiple cloning site for fusion to N-terminus of target protein

Antibodies from Article