Mapping Cellular Reprogramming via Pooled Overexpression Screens with Paired Fitness and Single-Cell RNA-Sequencing Readout.

Parekh U, Wu Y, Zhao D, Worlikar A, Shah N, Zhang K, Mali P
Cell Syst. 2018 Nov 12. pii: S2405-4712(18)30433-2. doi: 10.1016/j.cels.2018.10.008. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
120426	EF1a_mCherry_P2A_Hygro_Barcode	Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120427	EF1a_ASCL1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120428	EF1a_ASCL3_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ASCL3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120429	EF1a_ASCL4_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ASCL4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120430	EF1a_ASCL5_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120432	EF1a_CDX2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express CDX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120433	EF1a_CRX_P2A_Hygro_Barcode	Barcoded lentiviral vector to express CRX in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120434	EF1a_ERG_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ERG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120435	EF1a_ESRRG_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ESRRG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120436	EF1a_ETV2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120437	EF1a_FLI1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express FLI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120438	EF1a_FOXA1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express FOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120439	EF1a_FOXA2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express FOXA2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120440	EF1a_FOXA3_P2A_Hygro_Barcode	Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120441	EF1a_FOXP1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express FOXP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120442	EF1a_GATA1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express GATA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120444	EF1a_GATA4_P2A_Hygro_Barcode	Barcoded lentiviral vector to express GATA4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120445	EF1a_GATA6_P2A_Hygro_Barcode	Barcoded lentiviral vector to express GATA6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120447	EF1a_HAND2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express HAND2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120448	EF1a_HNF1A_P2A_Hygro_Barcode	Barcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120450	EF1a_HNF4A_P2A_Hygro_Barcode	Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120451	EF1a_HOXA1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120452	EF1a_HOXA10_P2A_Hygro_Barcode	Barcoded lentiviral vector to express HOXA10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120453	EF1a_HOXA11_P2A_Hygro_Barcode	Barcoded lentiviral vector to express HOXA11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120454	EF1a_HOXB6_P2A_Hygro_Barcode	Barcoded lentiviral vector to express HOXB6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120455	EF1a_KLF4_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120456	EF1a_LHX3_P2A_Hygro_Barcode	Barcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120457	EF1a_LMX1A_P2A_Hygro_Barcode	Barcoded lentiviral vector to express LMX1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120458	EF1a_MEF2C_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MEF2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120459	EF1a_MESP1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MESP1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120460	EF1a_MITF_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120461	EF1a_MYC_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120462	EF1a_MYCL_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120463	EF1a_MYCN_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120464	EF1a_MYOD1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120465	EF1a_MYOG_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120466	EF1a_NEUROD1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120467	EF1a_NEUROG1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express NEUROG1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120468	EF1a_NEUROG3_P2A_Hygro_Barcode	Barcoded lentiviral vector to express NEUROG3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120469	EF1a_NRL_P2A_Hygro_Barcode	Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120470	EF1a_ONECUT1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express ONECUT1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120471	EF1a_OTX2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120472	EF1a_PAX7_P2A_Hygro_Barcode	Barcoded lentiviral vector to express PAX7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120473	EF1a_POU1F1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express POU1F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120474	EF1a_POU5F1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120475	EF1a_RUNX1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express RUNX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120476	EF1a_SIX1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120477	EF1a_SIX2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SIX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120478	EF1a_SNAI2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SNAI2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120479	EF1a_SOX10_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120480	EF1a_SOX2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SOX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120481	EF1a_SOX3_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120482	EF1a_SPI1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SPI1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120483	EF1a_SPIB_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120484	EF1a_SPIC_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SPIC in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120485	EF1a_SRY_P2A_Hygro_Barcode	Barcoded lentiviral vector to express SRY in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120486	EF1a_TBX5_P2A_Hygro_Barcode	Barcoded lentiviral vector to express TBX5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120487	EF1a_TFAP2C_P2A_Hygro_Barcode	Barcoded lentiviral vector to express TFAP2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120488	EF1a_KLF1_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120489	EF1a_KLF2_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120490	EF1a_KLF3_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120491	EF1a_KLF5_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120492	EF1a_KLF6_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120493	EF1a_KLF7_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF7 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120494	EF1a_KLF8_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120495	EF1a_KLF9_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120496	EF1a_KLF10_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120497	EF1a_KLF11_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF11 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120498	EF1a_KLF12_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF12 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120499	EF1a_KLF13_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120500	EF1a_KLF14_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120501	EF1a_KLF15_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120502	EF1a_KLF16_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120503	EF1a_KLF17_P2A_Hygro_Barcode	Barcoded lentiviral vector to express KLF17 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120504	EF1a_MYC deltaMBI_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120505	EF1a_MYC deltaMBII_P2A_Hygro_Barcode	Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120506	EF1a_MYC deltaNLS_P2A_Hygro_Barcode	Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120507	EF1a_MYC deltab_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120508	EF1a_MYC deltaHLH_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC deltaHLH in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120509	EF1a_MYC deltaLZ_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120510	EF1a_MYC deltaNTD_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120511	EF1a_MYC deltaCTD_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120512	EF1a_MYC Glu39Ala_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120513	EF1a_MYC Thr58Ala_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
120514	EF1a_MYC Ser62Ala_P2A_Hygro_Barcode	Barcoded lentiviral vector to express MYC Ser62Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
135003	EF1a_mCherry_P2A_Hygro	Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.