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CRISPR-interference-based modulation of mobile genetic elements in bacteria.

Nyerges A, Balint B, Cseklye J, Nagy I, Pal C, Feher T
Synth Biol (Oxf). 2019;4(1):ysz008. doi: 10.1093/synbio/ysz008. Epub 2019 Mar 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120424pCRISThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3, IS5, and IS150.
120425pCRISP_ISThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.

Antibodies from Article