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Knockdown of the ADHD Candidate Gene Diras2 in Murine Hippocampal Primary Cells.

Grunewald L, Chiocchetti AG, Weber H, Scholz CJ, Schartner C, Freudenberg F, Reif A
J Atten Disord. 2019 Jan 9:1087054718822129. doi: 10.1177/1087054718822129. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120391pAAV-Linker-hSyn::mCherry.3xFLAG-WPREpAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter
120392pAAV-U6-hSyn::mCherry.3xFLAG-WPREpAAV plasmid with BspQI sites for insertion of target specific shRNAs in frame of the murine U6 promoter and co expressing mCherry.3XFLAG under the hSyn promoter
120393pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPREpAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter
120394pAAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPREpAAV plasmid expressing Diras2 shRNA2 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter
120395pAAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPREpAAV plasmid expressing scrambled shRNA under the U6 promoter and mCherry.3xFLAG under the hSyn promoter

Antibodies from Article