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The interaction between ORF18 and ORF30 is required for late gene expression in Kaposi's sarcoma-associated herpesvirus.

Castaneda AF, Glaunsinger BA
J Virol. 2018 Oct 10. pii: JVI.01488-18. doi: 10.1128/JVI.01488-18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120372ORF18-3xFLAG pCDNA4Expresses KSHV ORF18 with Cterm 3xFLAG tag in mammalian cells
120374UL79-3xFLAG pCDNA4Expresses HCMV UL79 with Cterm 3xFLAG tag in mammalian cells
120375UL91-2xStrep pCDNA4Expresses HCMV UL91with Cterm 2xStrep tag in mammalian cells
1203762xStrep-ORF34 pCDNA4Expresses KSHV ORF34 with Nterm 2xStrep tag in mammalian cells
120377K8.1 Pr pGL4.16Firefly luciferase reporter with KSHV K8.1 late gene promoter
120378ORF57 Pr pGL4.16Firefly luciferase reporter with KSHV ORF57 early gene promoter
129742ORF24-2xStrep pCDNA4Expresses KSHV ORF24 with Cterm 2xStrep tag in mammalian cells
129743ORF30-2xStrep pCDNA4Expresses KSHV ORF30 with Cterm 2xStrep tag in mammalian cells
129744ORF31-2xStrep pCDNA4Expresses KSHV ORF31 with Cterm 2xStrep tag in mammalian cells
129746pLVX-TetOneZeo-ORF18(WT)-3xFLAGDox inducible expression of KSHV ORF18-3xFLAG in mammalian cells
129758pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAGDox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells
129759UL79(E48A_L49A)-3xFLAG pCDNA4Expresses CMV UL79 (E48A_L49A) with Cterm 3xFLAG tag in mammalian cells
129777ORF18(L29A)-3xFLAG pCDNA4Expresses KSHV ORF18 (L29A) with Cterm 3xFLAG tag in mammalian cells
129778ORF18(E36A)-3xFLAG pCDNA4Expresses KSHV ORF18 (E36A) with Cterm 3xFLAG tag in mammalian cells
129779ORF18(L37A)-3xFLAG pCDNA4Expresses KSVH ORF18 (L37A) with Cterm 3xFLAG tag in mammalian cells
129780ORF18(N63A)-3xFLAG pCDNA4Expresses KSHV ORF18 (N63A) with Cterm 3xFLAG tag in mammalian cells
129781ORF18(G65A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G65A) with Cterm 3xFLAG tag in mammalian cells
129782ORF18(L72A)-3xFLAG pCDNA4Expresses KSHV ORF18 (L72A) with Cterm 3xFLAG tag in mammalian cells
129783ORF18(R74A)-3xFLAG pCDNA4Expresses KSHV ORF18 (R74A) with Cterm 3xFLAG tag in mammalian cells
129784ORF18(K75A)-3xFLAG pCDNA4Expresses KSHV ORF18 (K75A) with Cterm 3xFLAG tag in mammalian cells
129785ORF18(W81A)-3xFLAG pCDNA4Expresses KSHV ORF18 (W81A) with Cterm 3xFLAG tag in mammalian cells
129786ORF18 (Y85A)-3xFLAG pCDNA4Expresses KSHV ORF18 (Y85A) with Cterm 3xFLAG tag in mammalian cells
129787ORF18(L107A)-3xFLAG pCDNA4Expresses KSHV ORF18 (L107A) with Cterm 3xFLAG tag in mammalian cells
129795ORF18(G130A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G130A) with Cterm 3xFLAG tag in mammalian cells
129796ORF18(G145A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G145A) with Cterm 3xFLAG tag in mammalian cells
129798ORF18(N146A)-3xFLAG pCDNA4Expresses KSHV ORF18 (N146A) with Cterm 3xFLAG tag in mammalian cells
129799ORF18(L151A)-3xFLAG pCDNA4Expresses KSHV ORF18 (L151A) with Cterm 3xFLAG tag in mammalian cells
129800ORF18(G152A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G152A) with Cterm 3xFLAG tag in mammalian cells
129801ORF18(R158A)-3xFLAG pCDNA4Expresses KSHV ORF18 (R158A) with Cterm 3xFLAG tag in mammalian cells
129802ORF18(L159A)-3xFLAG pCDNA4Expresses KSHV ORF18 (L159A) with Cterm 3xFLAG tag in mammalian cells
129803ORF18(W170)-3xFLAG pCDNA4Expresses KSHV ORF18 (W170A) with Cterm 3xFLAG tag in mammalian cells
129804ORF18(G171A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G171A) with Cterm 3xFLAG tag in mammalian cells
129805ORF18(E176A)-3xFLAG pCDNA4Expresses KSHV ORF18 (E176A) with Cterm 3xFLAG tag in mammalian cells
129806ORF18(R180A)-3xFLAG pCDNA4Expresses KSHV ORF18 (R180A) with Cterm 3xFLAG tag in mammalian cells
129807ORF18(L191A)-3xFLAG pCDNA4Expresses KSHV ORF18 (L191A) with Cterm 3xFLAG tag in mammalian cells
129808ORF18(G214A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G214A) with Cterm 3xFLAG tag in mammalian cells
129809ORF18(G228A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G228A) with Cterm 3xFLAG tag in mammalian cells
129810ORF18(E36A_L37A)-3xFLAG pCDNA4Expresses KSHV ORF18 (E36A_L37A) with Cterm 3xFLAG tag in mammalian cells
129811ORF18(R74A_K75A)-3xFLAG pCDNA4Expresses KSHV ORF18 (R74A_K75A) with Cterm 3xFLAG tag in mammalian cells
129812ORF18(G145A_N146A)-3xFLAG pCDNA4Expresses KSHV ORF18 (G145A_N146A) with Cterm 3xFLAG tag in mammalian cells
129813ORF18(L151A_G152A)-3xFLAG pCDNA4Expresses KSHV ORF18 (L151A_G152A) with Cterm 3xFLAG tag in mammalian cells
129814ORF18(R158A_L159A)-3xFLAG pCDNA4Expresses KSHV ORF18 (R158A_L159A) with Cterm 3xFLAG tag in mammalian cells
129815ORF18(W170A_G171A)-3xFLAG pCDNA4Expresses KSHV ORF18 (W170A_G171A) with Cterm 3xFLAG tag in mammalian cells

Antibodies from Article