A Functional Mini-Integrase in a Two-Protein-type V-C CRISPR System.
Wright AV, Wang JY, Burstein D, Harrington LB, Paez-Espino D, Kyrpides NC, Iavarone AT, Banfield JF, Doudna JA
Mol Cell. 2019 Feb 21;73(4):727-737.e3. doi: 10.1016/j.molcel.2018.12.015. Epub 2019 Jan 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120364 | pET-3a-His-MBP-TEV-VC_Cas1_WT | Expresses wildtype V-C Cas1 |
| 120365 | pET-3a-His-MBP-TEV-VD_Cas1_WT | Expresses wildtype V-D Cas1 |
| 120366 | pVC-CRISPR1 | Plasmid containing shortened version of upstream V-C CRISPR array for integration assays |
| 120368 | pVC_CRISPR_Cat | Reporter plasmid for full-site selection assay |
| 120369 | pVC-CRISPR | Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library |
| 120370 | pVD_CRISPR | Plasmid containing shortened version of V-D CRISPR array for integration assays |