OVOL1 Influences the Determination and Expansion of iPSC Reprogramming Intermediates.
Kagawa H, Shimamoto R, Kim SI, Oceguera-Yanez F, Yamamoto T, Schroeder T, Woltjen K
Stem Cell Reports. 2018 Dec 28. pii: S2213-6711(18)30527-7. doi: 10.1016/j.stemcr.2018.12.008.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120352 | PB-TAC-Cas9 | piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed. |
| 120353 | PB-TAC-OKMS-Cas9 | piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed. |
| 120354 | PB-TAC-OK+9MS-Cas9 | piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed. |
| 120355 | PB-TAC-KRAB-dCas9 | piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed. |
| 120356 | PB-TAC-OKMS-KRAB-dCas9 | piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed. |
| 120357 | PB-TAC-OK+9MS-KRAB-dCas9 | piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed. |
| 120358 | PB-U6 | piggyBac transposon vector for sgRNA expression. |
| 120359 | pENTR-mOvol1 | Gateway entry vector containing mouse Ovol1. |
| 120360 | PB-TAB-mOvol1 | piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion). |