Directed evolution of a small-molecule-triggered intein with improved splicing properties in mammalian cells.
Peck SH, Chen I, Liu DR
Chem Biol. 2011 May 27;18(5):619-30. doi: 10.1016/j.chembiol.2011.02.014.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120304 | p3x-Flag Gli3 30R3-1 | Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 30C |
| 120305 | p3x-Flag Gli3 37R3-2 | Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 37C |