Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins.
Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Borner K, Eils R, Grimm D, Niopek D
Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120293 | AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E) | AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold |
| 120294 | AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites) | AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites) |
| 120295 | AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites | AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites |
| 120296 | pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold) | AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold) |
| 120297 | AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites) | AAV vector for expression of AcrIIA4 (no miR binding sites, control vector) |
| 120298 | AAV CMV-driven AcrIIA4-2xmiR-122 target sites | AAV vector for expression of AcrIIA4 with two miR-122 binding sites |
| 120299 | AAV CMV-driven AcrIIA4-2xmiR-1 target sites | AAV vector for expression of AcrIIA4 with two miR-1 binding sites |
| 120300 | AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites) | AAV vector for expression of AcrIIC1 (no miR binding sites, control vector) |
| 120301 | AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites) | AAV vector for expression of AcrIIC3 (no miR binding sites, control vector) |
| 120302 | AAV CMV-driven AcrIIC1-2xmiR-122 target sites | AAV vector for expression of AcrIIC1 with two miR-122 binding sites |
| 120303 | AAV CMV-driven AcrIIC3-2xmiR-122 target sites | AAV vector for expression of AcrIIC3 with two miR-122 binding sites |