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Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast.

Despres PC, Dube AK, Nielly-Thibault L, Yachie N, Landry CR
G3 (Bethesda). 2018 Oct 3;8(10):3163-3171. doi: 10.1534/g3.118.200461. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120263pDYSCKOEmpty backbone for cloning any gRNA to be used with the canavanine co-selection system
120264pDYSCKO-ADE1pDYSCKO canavanine co-selection vector with a guide targeting the ADE1 locus

Antibodies from Article