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Double UP: A Dual Color, Internally Controlled Platform for in utero Knockdown or Overexpression.

Taylor RJ, Carrington J, Gerlach LR, Taylor KL, Richters KE, Dent EW
Front Mol Neurosci. 2020 May 20;13:82. doi: 10.3389/fnmol.2020.00082. eCollection 2020. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
120261Double UP sfGFP to mScarletNatively produces sfGFP, in the presence of Cre will be recombined to produce mScarlet.
120262Double UP mClover3 to mScarletNatively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.
125134Double UP mNeon to mScarletNatively produces mNeon, in the presence of Cre will be recombined to produce mScarlet.
125135Double UP mNeon to mScarlet P2A Rac1-wtNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged WT Rac1.
125136Double UP mNeon to mScarlet Rac1-V12Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).
125137Double UP mNeon to mScarlet Rac1-N17Natively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-N17 (Dominant Negative).
125138Double UP Halotag to mScarletNatively produces Halotag, in the presence of Cre will be recombined to produce mScarlet.
125139Double UP mScarlet to mNeonNatively produces mScarlet, in the presence of Cre will be recombined to produce mNeon.
141111Double UP mNeon to mScarlet FRTNatively produces mNeonGreen, in the presence of Flp or Flpe will be recombined to produce mScarlet.

Antibodies from Article