Refactoring the Cryptic Streptophenazine Biosynthetic Gene Cluster Unites Phenazine, Polyketide, and Nonribosomal Peptide Biochemistry.
Bauman KD, Li J, Murata K, Mantovani SM, Dahesh S, Nizet V, Luhavaya H, Moore BS
Cell Chem Biol. 2019 Feb 15. pii: S2451-9456(19)30038-8. doi: 10.1016/j.chembiol.2019.02.004.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120229 | pCAP-BAC | TAR cloning in yeast and low-copy maintenance of large constructs in E. coli |
| 120230 | pJZ001 | Contains intB13 integrase casette for knock-in to pCAP-BAC backbone |
| 120231 | pJZ002 | CRISPR-cas9 targeting in E. coli with ampicillin resistance |
| 120232 | pCAP03-sp44/p21 | Carries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring. |
| 120233 | pCAP03-act1p | Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring. |
| 120234 | pCAP03-ermE*p | Carries promoter cassette containing ermE*p promoter and ampicillin resistance gene that can be amplified with homology sequence for refactoring. |