Christopher Reisch Lab: pCas9CR4 variants for increased targeting space
Unpublished
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
120224 | pCas9CR4-VQR | pCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015 |
120225 | pCas9CR4-NG | pCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al. |
120227 | pCas9CR5 | pCas9CR4 with stop codon to prevent translation of ssrA degradation tag |
120228 | pCas9CR5-NG | pCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al. |