Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning.
Guardia CM, De Pace R, Sen A, Saric A, Jarnik M, Kolin DA, Kunwar A, Bonifacino JS
PLoS Biol. 2019 May 17;17(5):e3000279. doi: 10.1371/journal.pbio.3000279. eCollection 2019 May.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 120163 | mCh-KIF5A*-strep | Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin |
| 120164 | mCh-KIF5B*-strep | Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin |
| 120165 | mCh-KIF1A*-strep | Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin |
| 120166 | mCh-KIF13A*-strep | Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin |
| 120167 | mCh-KIF17*-strep | Expresses a chimera of mCherry (fluorescent tag), a KIF17-based anterograde motor and streptavidin |
| 120168 | mCh-BICD2*-strep | Expresses a chimera of mCherry (fluorescent tag), a BICD2-based adaptor and streptavidin |
| 120169 | Strep-KIFC1*-mCh | Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag) |
| 120170 | HA-KIF5B*-strep | Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin |
| 120171 | Strep-KIFC1*-HA | Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag |
| 120172 | LAMP1-SBP-GFP | Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag) |
| 120173 | TOM20*-SBP-GFP | Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag) |
| 120174 | PEX3*-SBP-GFP | Expresses a chimera of the peroxisomal-targeting signal of PEX3, SBP tag and GFP (fluorescent tag) |
| 120175 | GFP-SBP-VAP-A* | Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A |