CRISPR-Cas12a-assisted PCR tagging of mammalian genes.

Fueller J, Herbst K, Meurer M, Gubicza K, Kurtulmus B, Knopf JD, Kirrmaier D, Buchmuller BC, Pereira G, Lemberg MK, Knop M
J Cell Biol. 2020 Jun 1;219(6). pii: 151766. doi: 10.1083/jcb.201910210. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
124791	pMaCTag-Z19	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mCherry-mNeonGreen
124790	pMaCTag-Z07	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreen
124789	pMaCTag-P19	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen
124788	pMaCTag-P07	Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeonGreen
124787	pMaCTag-19	Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen
124786	pMaCTag-07	Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen
120075	pMaCTag-Z32	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with S-Tag
120074	pMaCTag-Z31	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5
120073	pMaCTag-Z30	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with VSV-G
120072	pMaCTag-Z29	Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with Strep-II