Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites.
Liu Y, Cao Z, Wang Y, Guo Y, Xu P, Yuan P, Liu Z, He Y, Wei W
Nat Biotechnol. 2018 Nov 5. pii: nbt.4283. doi: 10.1038/nbt.4283.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119976 | pLenti-sgRNA(lib)-puro | Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences. |
| 119977 | Splicing-targeting CRISPR-Cas9 library for human lncRNAs | The CRISPR sgRNA library was designed to target the 5' or 3' splice sites of lncRNAs in genome-scale, in order to knockout the targeted lncRNAs. |