Single-Molecule Imaging of mRNA Localization and Regulation during the Integrated Stress Response.

Wilbertz JH, Voigt F, Horvathova I, Roth G, Zhan Y, Chao JA
Mol Cell. 2019 Jan 4. pii: S1097-2765(18)31036-0. doi: 10.1016/j.molcel.2018.12.006. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

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ID	Plasmid	Purpose
119950	phage UbiC G3BP1-GFP-GFP	Lentiviral vector expressing G3BP1-GFP fusion protein
119949	phage UbiC G3BP1-SNAP	Lentiviral vector expressing G3BP1-SNAP fusion protein
119948	phage UbiC SNAP-DDX6	Lentiviral vector expressing SNAP-DDX6 fusion protein
119947	phage UbiC tagRFP-T-DDX6	Lentiviral vector expressing tagRFP-T-DDX6 fusion protein
119946	pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA	Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
119945	pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA	Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats