Live-cell imaging reveals enhancer-dependent Sox2 transcription in the absence of enhancer proximity.
Alexander JM, Guan J, Li B, Maliskova L, Song M, Shen Y, Huang B, Lomvardas S, Weiner OD
Elife. 2019 May 24;8. pii: 41769. doi: 10.7554/eLife.41769.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119901 | pCAGGS-Bxb1o_nlsFlag | Expresses Bxb1 integrase in mammalian cells |
| 119903 | pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP | Bxb1 integration plasmid for cuO array with Puro selection cassette |
| 119906 | epB_UbC_CymRV5-nls-GFP_DEx2 | ePiggyBac mammalian expression plasmid for CymR-GFP fusion |
| 119907 | epB_UbC_CymRV5-nls-Halox2_DEx4 | ePiggyBac mammalian expression plasmid for CymR-Halo fusion |
| 119908 | epB_UbC_tdMS2cp-tagRFP-Tx2 | ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion |
| 119909 | epB_CAG_TetRFlag-nls-tdTom_DEx4 | ePiggyBac mammalian expression plasmid for TetR-tdTom fusion |
| 119910 | epB_CAG_TetRFlag-nls-GFPx2_DEx2 | ePiggyBac mammalian expression plasmid for TetR-GFP fusion |