A Compact, High-Accuracy Cas9 with a Dinucleotide PAM for In Vivo Genome Editing.
Edraki A, Mir A, Ibraheim R, Gainetdinov I, Yoon Y, Song CQ, Cao Y, Gallant J, Xue W, Rivera-Perez JA, Sontheimer EJ
Mol Cell. 2018 Dec 18. pii: S1097-2765(18)31033-5. doi: 10.1016/j.molcel.2018.12.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119923 | Nme2Cas9_pCDest | Nme2Cas9 CMV-driven mammalian expression plasmid |
| 119924 | Nme2Cas9_AAV | All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette |
| 119925 | Nme2Cas9_pMCSG7 | Nme2Cas9 bacterial expression plasmid |
| 119926 | Nme2sgRNA_pLKO | Nme2Cas9 U6-driven sgRNA cassette |
| 193190 | pSFFV-dNme2Cas9 | Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2) |