A Network of Noncoding Regulatory RNAs Acts in the Mammalian Brain.
Kleaveland B, Shi CY, Stefano J, Bartel DP
Cell. 2018 Jul 12;174(2):350-362.e17. doi: 10.1016/j.cell.2018.05.022. Epub 2018 Jun 7.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128744 | pcDNA3.1-mCherry | Expresses mCherry in mammalian cells |
| 128746 | pcDNA3.1-mCherry-CyrCR1.mutant | Expresses mCherry in mammalian cells. ~200 nt of Cyrano with 5 point mutations in miR-7 site inserted in mCherry 3'UTR. |
| 128747 | pcDNA3.1-mCherry-CyrCR1.miR16 | Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-16 site inserted in mCherry 3'UTR. |
| 128748 | pcDNA3.1-mCherry-CyrCR1.miR17 | Expresses mCherry in mammalian cells. ~200 nt of Cyrano with miR-7 site replaced by miR-17 site inserted in mCherry 3'UTR. |
| 128749 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.Control | Expresses control gRNA |
| 128755 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO | Expresses CYRANO gRNA for CRISPRi |
| 128756 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1 | Expresses PAPD4 gRNA for CRISPRi |
| 128757 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2 | Expresses PAPD4 gRNA for CRISPRi |
| 128758 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-3 | Expresses PAPD4 gRNA for CRISPRi |
| 128760 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.PARN-2 | Expresses PARN gRNA for CRISPRi |
| 128762 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1 | Expresses DIS3L2 gRNA for CRISPRi |
| 128763 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-2 | Expresses DIS3L2 gRNA for CRISPRi |
| 128764 | pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-3 | Expresses DIS3L2 gRNA for CRISPRi |
| 128768 | pcDNA3.1-mCherry-CyrCR1 | Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site. |