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Targeted m(6)A Reader Proteins To Study Epitranscriptomic Regulation of Single RNAs.

Rauch S, He C, Dickinson BC
J Am Chem Soc. 2018 Sep 26;140(38):11974-11981. doi: 10.1021/jacs.8b05012. Epub 2018 Sep 18. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
119855Cmv d0 dPspCas13b-GGS-NYTHDF1Cmv d0 dPspCas13b-GGS-NYTHDF1 fusion protein (full N-terminal domain of YTHDF1, C-terminal YTH domain omitted)
119856Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)
119857Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)
119858Cmv d0 dPspCas13b-GGS-SunCmv d0 dPspCas13b-GGS-Sun no-fusion construct
119859hU6 promoter PspCas13b gRNA dual luciferase reporter 3’UTRgRNA plasmid with hU6 promoter PspCas13b gRNA dual luciferase reporter

Antibodies from Article