Improved sgRNA design in bacteria via genome-wide activity profiling.
Guo J, Wang T, Guan C, Liu B, Luo C, Xie Z, Zhang C, Xing XH
Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113138 | E.coli_GW-sgRNA_Lib5 | Genome-wide gRNA library for E. coli, sub5 |
| 113147 | LibTest | Customized sgRNA library targeting 86 E. coli genes |
| 113150 | pCas9-J23109 | Plasmid constitutively expressing Cas9 protein |
| 113151 | pdCas9-J23109 | Plasmid constitutively expressing dCas9 protein |
| 113152 | peSpCas9-J23109 | Plasmid constitutively expressing eSpCas9 protein |
| 113153 | peSpdCas9-J23109 | Plasmid constitutively expressing eSpdCas9 protein |
| 119701 | pCas9-J23113 | Expression of Cas9 with a weak promoter |