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A rapid and simple method of evaluating the dimeric tendency of fluorescent proteins in living cells using a truncated protein of importin alpha as fusion tag.

Nakagawa C, Nishimura S, Senda-Murata K, Sugimoto K
Biosci Biotechnol Biochem. 2012;76(2):388-90. doi: 10.1271/bbb.110677. Epub 2012 Feb 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
119718pEGFP-imp αExpresses EGFP-tagged human importinα in mammalian cells
119719pmCherry-imp αExpresses mCherry-tagged human importinα in mammalian cells
119720mOrange-imp αExpresses mOrange-tagged human importinα in mammalian cells
200082pmKO-imp αExpresses mKO-tagged human importinα in mammalian cells

Antibodies from Article