Co-assembly of SecYEG and SecA fully restores the properties of the native translocon.
Bariya P, Randall LL
J Bacteriol. 2018 Oct 1. pii: JB.00493-18. doi: 10.1128/JB.00493-18.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119305 | pAL832 | precusor outer membrane protein A, ompA, truncated after amino acid 176 |
| 119306 | pAL800 | precusor outer membrane protein A, ompA, with amino acids 1 through 171 deleted and mutations G244C and C290S |
| 119697 | pAL829 | precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C |
| 119721 | pAL942 | precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine |
| 119748 | pAL950 | precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine |
| 119853 | pAL928 | precusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427C |
| 119860 | pAL612 | precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine |
| 119862 | pAL725 | precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C |