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Clonal analysis by tunable CRISPR-mediated excision.

Gilles AF, Schinko JB, Schacht MI, Enjolras C, Averof M
Development. 2019 Jan 4;146(1). pii: dev.170969. doi: 10.1242/dev.170969. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
119063pBac(3xP3-gTc’v; Tc-hsp68:Cre)Expression of Cre recombinase in Tribolium castaneum
119064pBac(3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry)Cre/lox flip-out construct for marking cell clones in Tribolium castaneum
119066pBac(3xP3-gTc’v; Valcyrie.LR)Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)
119068pBac(3xP3-gTc’v; Valcyrie.Uni)Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (low)

Antibodies from Article