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A General Strategy for the Semisynthesis of Ratiometric Fluorescent Sensor Proteins with Increased Dynamic Range.

Xue L, Prifti E, Johnsson K
J Am Chem Soc. 2016 Apr 27;138(16):5258-61. doi: 10.1021/jacs.6b03034. Epub 2016 Apr 15. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
119043pBAD-MTX_SnifitPlasmid encoding the bacterial expression of the control Snifit-MTX (FRET semi-synthetic biosensor)
119044pBAD-MTX-u23_SnifitPlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)
119045pBAD-MTX-u36_SnifitPlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
119046pBAD-MTX-u51_SnifitPlasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)

Antibodies from Article