Design of fast proteolysis-based signaling and logic circuits in mammalian cells.
Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118966 | FRB_nPPVp | Expresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domain |
| 118967 | FKBP_cPPVp | Expresses C-terminus of plum pox virus protease (PPVp) fused to the FK-506 binding protein (FKBP) |
| 118968 | FKBP_cSbMVp | Expresses C-terminus of soybean mosaic virus protease (SbMVp) fused to the FK-506 binding protein (FKBP) |
| 118969 | FRB_nSbMVp | Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused to FKBP-rapamycin binding (FRB) domain |
| 118970 | FRB_nSuMMVp | Expresses N-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain |
| 119182 | FKBP_cSuMMVp | Expresses C-terminus of sunflower mild mosaic virus protease (SuMMVp) fused to the FK-506 binding protein (FKBP) |
| 119207 | cycLuc_TEVS | Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity) |
| 119208 | cycLuc_PPVs | Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity) |
| 119209 | cycLuc_SbMVs | Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity) |
| 119210 | cycLuc_SuMMVs | Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity) |
| 119211 | PYL1_nPPVp | Expresses N-terminus of plum pox virus protease (PPVp) fused to PYL1-abscisic acid responsive protein domain |
| 119212 | ABI_cPPVp | Expresses C-terminus of plum pox virus protease (PPVp) fused to the ABI-abscisic acid responsive protein domain |
| 119213 | PYL1_nTEVp | Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain |
| 119214 | ABI_cTEVp | Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain |
| 119299 | nLuc_AP4_TEVs_P3mS | Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP4 connected with autoinhibitory coil P3mS and TEV cleavage site in antiparallel harpin linker/for SPOC logic |
| 119300 | AP4mS_PPVs_P3_cLuc | Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic |
| 119302 | nLuc_AP10_PPVs_P9mS | Expresses N-terminus of split luciferase fused to antiparallel coiled-coil AP10 connected with autoinhibitory coil P9mS and PPVs cleavage site in antiparallel harpin linker/for SPOC logic |
| 119303 | P9_TEVs_cLuc | Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic |