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Gene editing vectors for studying nicotinic acetylcholine receptors in cholinergic transmission.

Peng C, Yan Y, Kim VJ, Engle SE, Berry JN, McIntosh JM, Neve RL, Drenan RM
Eur J Neurosci. 2018 May 19. doi: 10.1111/ejn.13957. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128337pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128338pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128339pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128340pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128341pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128342pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128343pFB-U6-beta2 RD-1-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128344pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128345pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding gRNA sequence for loss-of-function indels
128346pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding control gRNA for CRISPR gRNAs listed above
128347pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pApAAV encoding control gRNA for CRISPR gRNAs listed above

Antibodies from Article