FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing.

Lemaitre RP, Bogdanova A, Borgonovo B, Woodruff JB, Drechsel DN
BMC Biotechnol. 2019 Mar 29;19(1):20. doi: 10.1186/s12896-019-0512-z. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
118912	pOCC94	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6
118911	pOCC93	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6
118910	pOCC92	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal eGFP-HIS6
118909	pOCC167	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal and C-terminal HIS6, cleavable with 3C protease
118908	pOCC219	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag
118907	pOCC218	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS10, cleavable with 3C protease and N-terminal YBBR tag
118906	pOCC111	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal sortase site (LPETGg) and HIS6
118905	pOCC110	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)
118904	pOCC114	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site
118903	pOCC193	shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C