Human tumor genomics and zebrafish modeling identify SPRED1 loss as a driver of mucosal melanoma.
Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
Science. 2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118840 | MiniCoopR U6:gRNA, mitfa:Cas9 | Targets a gene and expresses zebrafish mitfa specifically in zebrafish melanocytes |
| 118841 | MiniCoopR U6:gRNA p53, mitfa:Cas9 | Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes |
| 118842 | MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9 | Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes |
| 118843 | MiniCoopR U6:gRNA spred1, mitfa:Cas9 | Targets spred1 and expresses zebrafish mitfa specifically in zebrafish melanocytes |
| 118844 | MiniCoopR 2xU6:gRNA, mitfa:Cas9 | Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes |
| 118845 | MiniCoopR 2xU6:gRNA pten, mitfa:Cas9 | Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes |
| 118846 | MiniCoopR mitfa:BRAF | Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes |
| 118847 | MiniCoopR mitfa:NRAS | Expresses human NRAS Q61R mutant and zebrafish mitfa specifically in zebrafish melanocytes |
| 118848 | MiniCoopR mitfa:KIT L576P | Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes |
| 118849 | MiniCoopR mitfa:KIT K642E | Expresses human KIT K642E mutant and zebrafish mitfa specifically in zebrafish melanocytes |
| 118850 | MiniCoopR mitfa:EGFP | Expresses EGFP and zebrafish mitfa specifically in zebrafish melanocytes |
| 118851 | MiniCoopR mitfa:SPRED1 | Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytes |