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Low-cost detection of norovirus using paper-based cell-free systems and synbody-based viral enrichment.

Ma D, Shen L, Wu K, Diehnelt CW, Green AA
Synth Biol (Oxf). 2018;3(1):ysy018. doi: 10.1093/synbio/ysy018. Epub 2018 Sep 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118815pDM_T7_HisLacZomegaT7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.
118816pDM_noro_S2_lacZAT7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.
118817pDM_noro_S6_lacZAT7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.
118818pDM_noro_A1_lacZAT7 RNAP-driven expression of norovirus antisense orientation toehold switches (A1) with a lacZ alpha subunit reporter.
118819pDM_noro_A2_lacZAT7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.
118820pDM_noro_A2_lacZT7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ reporter.

Antibodies from Article